Colloidal gold Falun virus fecal rapid testing: an instant diagnostic tool for preventing and controlling diarrhea in infants and young children

Rotavirus is the leading pathogen of severe diarrhea in children under 5 years old worldwide, causing approximately 900000 child deaths annually, with developing countries accounting for 85% of deaths. High incidence areas include:

Southeast Asia/Sub Saharan Africa: In areas with weak sanitation conditions, the detection rate of rotavirus in children with diarrhea cases is 50% -60%, and the in-hospital mortality rate is 4.7% (2023 data);

Poor communities in South America: Water pollution has led to an outbreak of the epidemic, with an infection rate of over 70% among children under 5 years old.

 

Vulnerable populations and health hazards‌

High risk population:

Infants and young children aged 6 months to 2 years old: accounting for 80% of infection cases, with the highest risk of severe dehydration;

Children with immunodeficiency: The mortality rate after infection is 3-5 times higher than that of healthy children;

Childcare institutions/nursing homes: The outbreak rate of clustered infections is 30% -50%.

Health hazards:

Watery diarrhea and dehydration: Severe children with more than 10 bowel movements per day have a 40% risk of dehydration;

Secondary malnutrition: Repeated infections lead to a 15% increase in growth retardation rates (data from developing countries).

 

Route of transmission‌

Fecal oral transmission: accounting for 90% of infected cases, the virus is transmitted through contaminated water sources, food, or contact;

Environmental pollution: Rotavirus can survive in feces for 7-14 days and continue to spread after contaminating toys and clothing;

Occasional respiratory transmission: Animal experiments have confirmed the possibility of aerosol transmission, but it accounts for less than 5%.

Comparison of detection techniques and advantages of colloidal gold method‌

Testing method	Sensitivity	Specificity	Testing time	Applicable scenarios‌
Colloidal gold method	85%-90%	95%	10-15minutes	On site screening, rapid response to epidemic outbreaks
‌PCR	90%	99%	4-6hours	Laboratory classification and precise diagnosis
Virus cultivation	50%-60%	100%	7-14days	Research/Special Typing Needs

 

Core advantages of colloidal gold method:

Instant diagnosis: Results will be available within 15 minutes, assisting in early screening and control for childcare institutions and remote areas;

Zero threshold for operation: No need for equipment or professional personnel, can be tested at home;

Fecal sample suitability: For infants and young children with diarrhea, the detection rate is 30% higher than that of respiratory samples;

Outstanding cost-effectiveness: The cost of a single test is only 1/15 of PCR, making it suitable for large-scale screening.

 

Galenus Medical's Rotavirus Rapid Test Device for rotavirus is based on colloidal gold immunochromatography technology, which captures rotavirus antigen through a double antibody sandwich method and forms a color band; Support direct detection of fecal samples without centrifugation or pretreatment. Can be used in kindergartens and community hospitals to quickly identify the source of infection and block the transmission chain; Large scale screening in impoverished areas to reduce severe illness and mortality rates.